Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful analytical technique used to detect and quantify substances such as peptides, proteins, antibodies, and hormones. It leverages the specificity of antibodies to identify target antigens, making it an essential tool in diagnostics, research, and various fields of biotechnology.
How ELISA Works
The ELISA process typically involves the following steps:
- Coating: Antibodies are attached to the surface of microplate wells.
- Blocking: Any nonspecific binding sites are blocked to prevent false positives.
- Binding: The sample containing the antigen is added, allowing it to bind to the antibodies.
- Detection: A secondary antibody, linked to an enzyme, binds to the antigen.
- Substrate Addition: The enzyme reacts with a substrate to produce a measurable signal, usually a color change.
- Measurement: The signal is quantified using a microplate reader.
Types of ELISA
- Direct ELISA: The antigen is directly immobilized on the assay plate and detected with an enzyme-labeled antibody.
- Indirect ELISA: Involves two binding processes: the primary antibody and the enzyme-labeled secondary antibody.
- Sandwich ELISA: Utilizes a pair of antibodies – the capture antibody and the detection antibody.
- Competitive ELISA: The sample antigen competes with a reference antigen for binding to the antibody.
Why Choose InstaMed?
At InstaMed, we offer over 700 ELISA kits tailored to meet the diverse needs of research and clinical laboratories. Our extensive range includes kits for various applications, ensuring that you can find the right solution for your specific requirements.
Automation in ELISA
We also provide state-of-the-art automated instruments for ELISA testing, which enhance efficiency, accuracy, and throughput. Automation minimizes manual errors and allows for high-throughput screening, making it easier to handle large sample volumes.
Tailored Solutions for Every Lab
Whether you are a small research lab or a large clinical facility, InstaMed caters to your ELISA testing needs with precision and reliability. Our products are designed to support high-performance results, ensuring that you get the most accurate data for your studies or diagnostics.
Explore our comprehensive range of ELISA kits and automated instruments today and see how we can support your laboratory’s success.
For more detailed information about ELISA, you can visit https://instamed.ae/automated-elisa-analyzer/
Contact us to learn more about our offerings and how we can assist with your ELISA testing needs.
